Abstract: In response to the problems of restricted access to the public, lack of management personnel and insufficient professional capacity of the purchased large instruments, taking the Talos F200X transmission electron microscope as an example, a system based on custodian model was established under the custodian of the Analysis and Testing Center, which is shared by the research group and the Analysis and Testing Center. And the Analysis and Testing Center providing open sharing to both internal and external users. The operational management of the Talos F200X transmission electron microscope was briefly outlined, the common faults and solutions were discussed in detail, and the key points for routine maintenance were summarized to provide a reference for management personnel of similar instruments.