Study on the Spectorofluorimetric System of PM_O Hetoropoly Acid and Rhodamine B and Its Application

A new spectrofluorometric method for the determination of 0.3～30 μg/L P has been described. It is based on that PO₄^3-, MoO₄^2- and rhodamine B form a fluoresence ternary association complex in the medium of 0.08 mol/L H₂SO₄. The λ_ex and λ_em are 325 nm and 650 nm respectively. The results show that this method has high sensitivity, good selectivity and easy to operate. It has been applied to analysis of phosphorus in water, copper alloy and tea samples, with satisfactory results.