Studies on Chromatography Fingerprint(CFP) of Danggui by High-performance Liquid Chromatography

Objective: Study on chromatographic fingerprint of Danggui herbs. Method: High-performance liquid chromatography on a Hypersil ODS column with HOAc(1%)-methanol gradient elution,flow-rate of 1.0 mL/min,column temperature of 25℃,standard such as Danggui standard,acquisition wave range was 190～400 nm.Determined content of ferulic acid with PAD.Mobile phase: Methanol-1% HOAc(60:40,V/V),flow-rate of 0.7 mL/min,detector wave: 323 nm. Result: 22 common peaks were pointed out,5th peak was ferulic acid,their similarity of 11 samples were more than 90.0%. Conclusion: Handle method of samples was simple,stable and repeatable.The chromatographic fingerprint of Danggui samples could be used to aqueous solution section of them characterized chromatographic fingerprint.