Determination of Ginsenosides in Panax Japonicus C.A. Mey by HPLC

A HPLC method for determination of ginsenosides including R_g1、R_e、R_b1、R_b2、R_g2、R_d in panax japonicus C.A.Mey was reported. HPLC analysis was performed on a C₁₈ reversed-phase column (5 μm 、4.6 mm×250 mm)using mobile phase of acetonitrile and water mixture under gradient elution.Ginsenosides Rg1、Re、Rd in a bath of chilkusetsusaponin extract were tested .The contents of R_g1、R_e、R_d were 0.81% , 0.15%，2.99% respectively and the recovery was 93.46%~94.02%. Both sides relative standard deriations were less than 5%. This method is simple, sensitivity, reproducibility and can be used for the determination of ginsenosides in panax japonicus C.A.Mey by HPLC.