Determimation of Chrysoidin and Auramine O in Food by Solid Phase Extraction-UltraPerformance Liquid Chromatography-Mass Spectrometry

A method for the simultaneous determination of Chrysoidin and Auramine O in food by solid phase extraction-ultra performance liquid chromatography combined with tandem mass spectrometry has been developed. The sample extract was purified by C₁₈ solid-phase extraction column, the analysis can be accomplished within three minutes using this method. Detection limits (S/N=3)of Chrysoidin and Auramine O were 0.34 μg/kg and 1.28 μg/kg, limits of quantification (S/N=10) were 1.13 μg/kg and 4.27 μg/kg, respectively. Recoveries ranged from 80.1% to 95.3%.The method is quick, high sensitive, accurate and reliable and can meet the demands of qualitative and quantitative determinations of low content Chrysoidin and Auramine O in food.