Determination of Carbendazim, Thiabendazole and Methyl Thiophanate in Apple by High Performance Liquid Chromatography

A high performance liquid chromatographic(HPLC) method has been developed to determine the residues of carbendazim, thiabendazole and thiophanate methyl in apple. Samples were extracted with ethyl acetate, concentrated in a rotary evaporator, purged by nitrogen, added methanol to a constant volume and determined by HPLC with a diode array detector (DAD). The average spiked recoveries of carbendazim, thiabendazole and thiophanate methyl ranged from 87.7% to 118.7% , 72.8% to 80.3% and 64.0% to 66.8% at different concentrations, and the detection limits of carbendazim, thiabendazole and methyl thiophanate in apple samples were 0.134 mg/L, 0.230 mg/L, and 0.250 mg/L, respectively. The results showed the method is suitable for the determination of carbendazim, thiabendazole and methyl thiophanate in apple, and the residue of carbendazim in apple peel was 7.24×10^-2 mg/kg, thiabendazole and methyl thiophanate were not detected .These concentrations were lower than the national residual limit standards for carbendazim, thiabendazole and methyl thiophanate in apple.