Determination of Dissociation Constant and BindingConstant of Famciclovir

Both the dissociation constants of famciclovir obtained were 3.8 using ultraviolet spectrophotometry with the wavelengths at 225 nm and 305 nm. Bovine serum albumin was added to a Na₂HPO₄-KH₂PO₄ buffer solution (pH 7.0) with famciclovir. The binding constant was measurd by the double-reciprocal analysis. The results showed that bovine serum albumin and famciclovir formed a 1∶4 complex, and the binding constant obtained was 4.51×10⁶ L/mol. This method is simple and reliable.