Simultaneous Determination of Macrolide and Chloramphenicols Antibiotic Residues in Animal Derived Food by HPLC Method

An HPLC method for the simultaneous determination of residues of tylosin（TYL）,tilmicosin(TIL), chloramphenicol(CAP) and florfenicol(FF) in animal-derived food was developed. The muscle tissue samples were extracted by acetonitrile,then defatted with hexane, and cleaned up on a HLB SPE column. The analytes were separated on a reversed-phase C18column with the methanol-20 mmol/L NaH₂PO₄ solution (pH=3.0,containing 10% methanol), the UV detector was set at λ=275 nm. The standard curves were linear in the range of 0.1～20.0 mg/L for all analytes with correlation coefficients of 0.9970～0.9992.The detection limits for tylosin,tilmicosin, chloramphenicol(CAP), and florfenicol(FF),were 20 μg/kg, 32 μg/kg, 19 μg/kg, 16 μg/kg, respectively. The average recoveries of four analytes ranged from 75% to 87%. The relative standard deviations were between 1.35％～5.41％. The method can meet the requirement for the simultaneously determination of tylosin, tilmicosin, chloramphenicol(CAP), and florfenicol(FF) in beef products.