Determination of Free Amino Acids in Precious Substances of Anoectochilus Roxburghiiby Anion Chromatography-Integral Pulse Ampere

Objective:The qualitative and quantitative determination of free amino acids in precious medicinal material Anoectochilus roxburghii.Method: The anion chromatography-integral pulse ampere method is used in the qualitative and quantitative determination of free amino acids in precious medicinal material Anoectochilus roxburghii.Results: The limits of detection (IOD) for 17 amino acids fell between 0.23~3.37 pmol, the linearity range of peak area calibration curve (profile) was the order of 2-3 and RSDs for five determinations were 0.96%~4.2%.Conclusion: This method is applicable to the determination of free amino acids in the medicinal material Anoectochilus roxburghii with good results. And the recovery of dried Anoectochilus roxburghii was 88.9%~108.0%.