Simultaneous Determination of Fructose, Glucose and Sucrose in Red Jujube by High Performance Liquid Chromatography with Differential Detector

A method for the simultaneous determination of fructose,glucose and sucrose in red jujube was established by using a high performance liquid chromatograph with a differential detector. The average recoveries of the method for fructose, glucose and sucrose determination in spiked red jujube samples were 93.5%,91.8% and 89.8% with the variation coefficient ranged from 3.27%,2.34%,5.86% respectively.The results showed that the established method is simple and fast in the determination of fructose, and glucose, and sucrose in red jujube. The method detection limits were: fructose, 5.73×10^-3 mg/mL,glucose, 5.22×10^-3 mg/mL, sucrose, 3.59×10^-3mg/mL, the measured results meet the food testing requirements.